Stem cells on a scaffold​

Scaffolds are popular in biomedical applications due to their very high surface area and the possibility of growing microorganisms and cells. However, their high surface area is a challenging case for AFM topography study since there is a high chance of destroying the probe or the sample during navigation. 

Therefore, the AFM-in-SEM technique enabled us to localise to the region of interest using SEM and further provide a detailed surface topography study using AFM.

Measurement modes: F/z spectroscopy, Topography

LiteScope enables:

• Fast ROI identification and safe tip navigation
  
• SEM material contrast of cell/scaffold
  
• Cell morphology study – topography, membrane roughness, stiffness

Sample courtesy of Lucy Vojtová, CEITEC